Supporting Information for Antioxidative effects and mechanism study of bioactive peptides from defatted walnut (Juglans regia L.) meal hydrolysate Ji

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1 Supporting Information for Antioxidative effects and mechanism study of bioactive peptides from defatted walnut (Juglans regia L.) meal hydrolysate Jianyong Sheng a, Xiaoyu Yang a, Jitang Chen a, Tianhao Peng a, Xiquan Yin b, Wei Liu a, Ming Liang b*, Jiangling Wan a,* and Xiangliang Yang a a National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei , People s Republic of China b Joint Laboratory for The research of Modern Preparation Technology- Huazhong University of Science and Technology and Infinitus, Guangzhou, Guangdong , People s Republic of China *Corresponding author. Ming Liang, Tel: Fiona.Liang@infinitus-int.com * Corresponding author. Jiangling Wan, Tel: ; fax: wanjl@hust.edu.cn Jianyong Sheng and Xiaoyu Yang contributed equally to this work.

2 Table S1. Information of peptides with highest abundance ranking top15 in the de novo sequencing results of DWMH1 No. Sequences pi 1 VEGNLQVLRPR QLQVLRPR QLPR VNLVNQHKLPL LGLLPSFSNAPR LLANEPR NLPLL VVDSEGKWEL VLRGDA AVLDLSNHANQLD LVAVTVEDEL LSLLPSYQPTSP QNGAGFEWV LSTVNSQNLPLL TLMMSEEL

3 Table S2. Information of peptides with highest confidence ranking top15 in the de novo sequencing results of DWMH1 No. Sequences pi 16 LAGNPHQQQQN EAQFGQQHVSGAGQ HNLDTQTESDV SLLDTNNNANQLDQNPR NTNNNANQLDQNPR AGNDGFEYVTLK WSVWEQELEDR NSALYVPHWNLNAH QQRQQQGL QNPDDEFRPQGQ AELQVVDHLGQTV EQEEEESTGRMK QQERHHGQQQ TMFQQQSQHPAQPPR WSVWEQEL

4 Table S3. Information of peptides with highest abundance ranking top15 in the de novo sequencing results of DWMH2 No. Sequences pi 1 VEGNLQVLRPR DVARVALAP EPDVLALLQG VNLVNQHKLPL LGLLPSFSNAPR LLANEPR NLPLL VVDSEGKWEL VLRGDA LVAVTVEDEL RLDWVEA PEEVLALA LSTVNSQNLPLL QPDVTQVFEFQ VQLVDDNGDNVFDWV

5 Table S4. Information of peptides with highest confidence ranking top15 in the de novo sequencing results of DWMH2 No. Sequences pi 16 LAGNPHQQQQN HNLDTQTESDV AGNDGFEYVTLK AELQVVDHLGQTV MMRPDEDEQEGAGRQ DTNNNANQLDQNPR LAGNPDDEFTKQGPSQEYEQH DDDLREGQL LRHRADTQTESDV EQEEEESTGRMK QQRQQQGL SDALYVPHWARNAH WSVWEQELEDR LSTVNSQNLPLL LCVQQSGSNLFSGFDVCFL

6 Figure S1. MALDI-TOF mass spectrometry of (A) DWMH1 and (B) DWMH2. 5

7 (A) (B) (C) (D) (E) (F) (G) (H) Figure S2. HPLC chromatograms of peptides selected from DWMH. (A) P1: VEGNLQVLRPR, (B) P16: LAGNPHQQQQN, (C) P18: HNLDTQTESDV, (D) P21: AGNDGFEYVTLK, (E) P22: WSVWEQELEDR, (F) P24: QQRQQQGL, (G) P26: AELQVVDHLGQTV, (H) P27: EQEEEESTGRMK. 6

8 #810 RT: 8.04 AV: 1 NL: 2.90E7 F: FTMS + p ESI Full ms [ ] z= z= m/z (A) (B) (C) 12 Relative Abundance (D) (E) 7

9 #323 RT: 3.09 AV: 1 NL: 2.71E7 F: FTMS + p ESI Full ms [ ] z= z= m/z #1921 RT: AV: 1 NL: 6.22E7 F: FTMS + p ESI Full ms [ ] z= z= z= z= z=4 z=3 z= m/z (F) 13 Relative Abundance (G) 14_ Relative Abundance (H) Figure S3. Mass spectrums of peptides selected from DWMH. (A) P1: VEGNLQVLRPR, (B) P16: LAGNPHQQQQN, (C) P18: HNLDTQTESDV, (D) P21: AGNDGFEYVTLK, (E) P22: WSVWEQELEDR, (F) P24: QQRQQQGL, (G) P26: AELQVVDHLGQTV, (H) P27: EQEEEESTGRMK. 8

10 Figure S4. The different concentration of H 2 O 2 induced oxidative damage in SH-SY5Y cells. * p<0.05 vs the control group. 9

11 Figure S5. Protective effects of different concentration of peptides derived from DWMH or DWMH on H 2 O 2 induced oxidative damage in SH-SY5Y cells. * means had significant difference with the 150 μg/ml group, # means had significant difference with the 300 μg/ml group. 10